On the other hand, various methods of isolation of spermatids from testis are available 另一方面,已经报道了多种不同的方法可从睾丸中分离精子细胞。
This method of isolation, cultivation and purification of the synovial cells in vitro is proved to be simple and feasible 结论本研究建立了简单易行的滑膜细胞分离、培养和纯化方法。
To explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics 摘要研究人胎肝干细胞体外分离纯化方法,并对其进行初步鉴定及生物学特性分析。
Occurs as a white or nearly white, granular or crystalline powder and as a mixture of various states of hydration, up to the trihydrate, depending on the method of isolation 本品为白色结晶性或颗粒性粉末本品在沸水中极易溶解,在水中溶解,在无水乙醇氯仿或乙醚中不溶。
Thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro . the results showed below : 1 我们以小鼠为模型,运用组织化学、免疫荧光组织(细胞)化学、流式细胞仪分选方法(facs)以及分子生物学手段,研究了小鼠乳腺的发育规律:小鼠乳腺组织中类乳腺干细胞:小鼠乳腺细胞的分离、培养以及类乳腺干细胞的鉴定;小鼠类乳腺干细胞分化的潜能;小鼠乳腺类腺体体外短期培养富集类乳腺干细胞体系的优化等。研究结果表明:1
And introduced the information discover technology, which includes topology discovery, host scan and host os detection, etc . and is needed by the isolation technology . and then introduced the principal of end host isolation technology based on ospf, put forward two methods of isolation : configure ip address and configure the routing table . and the compared the use area and the speed of this two methods 然后介绍了末端隔离技术所需要的网络信息获取技术原理,包括拓扑发现和主机探测技术,接着介绍了基于ospf的末端隔离技术的原理,提出两种隔离方式:配置主机ip和配置路由表方式,并对比分析了这两种方式的使用范围和速度,介绍了隔离效果验证与对隔离节点访问的重定向技术。
Objective the origin and the nature of the hodgkin and reed-sternberg ( h / rs ) cell of hodgkin's lymphoma ( hl ) has been attracting a lot of medical researchers engaged in studying it, for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells . great improvement has been made since a new method of isolation of single h / rs cells from a frozen section had been set up by kuppers in 1994, and many studies have approved of the b-cell derivation of h / rs cell . lt has been reported that h / r-s cells might partly be originated from b-cell in our research before, but at the same time, we also found that only 18.8 % of h / rs cells express cd20, 31.3 % of immunoglobulin heavy chain ( igh ) rearrangement have been revealed 目的霍奇金淋巴瘤(hodgkin'slymphoma,hl)由于它的恶性肿瘤细胞?hrs细胞一般只占肿瘤组织的极少部分(不到1),且散在分布在背景细胞间,因此对于hrs细胞的来源和性质研究一直是人们探索的目标。1994年德国科隆大学kppers等发展了一种从冰冻组织切片上用显微操作仪挑取单个hrs细胞的显微切割方法进行hrs细胞基因分析后,人们对hrs细胞来源的研究有了突破性的进展,多数支持b细胞来源。我们的前期研究也支持部分hrs细胞为b细胞来源,但我们发现只有18.8的hrs细胞表达cd20,31.3的hl检测到igh基因重排克隆性条带。